Abstract—Biotechnological production of 2,3-butanediol ( BD), a value added chemical from wastes and excessive biomass is a promising and attractive alternative for traditional chemical synthesis. A BD concentration of 0.61% has been achieved form 2.35% lactose utilized after 96 hrs. of incubation period using deproteinated whey as substrate. This corresponds to BD production of 0.259 g/g lactose utilized. Adding of 50 mM acetate increases BD production to 0.84% from 2.3% lactose utilized corresponding to a yield of 0.365g/g lactose utilized. At solvent to feed ratio of 5:1 an overall mass transfer coefficient of 0.1554 x10-3 sec-1 and extraction of 84.47 % was achieved with glucose fermentation broth. When concentrated DPW broth was used for continuous countercurrent extraction higher Kda of 0.239 x10-3 sec-1 and extraction of 94 % and equilibrium stages of 1.346 was achieved.
Index Terms—2,3-Butanediol, butylene glycol, K.oxytoca, deproteinated whey, whey permeate, solvent extraction, countercurrent extraction.
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Cite: Dr. Sadhana Vishwakarma, "Countercurrent Extraction of 2,3-Butanediol," International Journal of
Chemical Engineering and Applications vol. 1, no. 2, pp. 147-150, 2010.
