Abstract—The strain improvement of Sporidiobolus johnsonii ATCC 20490 for improved production of Coenzyme Q10 (CoQ10) was undertaken. Ultraviolet (UV) rays and Ethyl Methane Sulphonate (EMS) were used as a mutagenic agent to generate mutant strains, out of which the desired mutants having resistance to high temperature and various inhibitors like atorvastatin, ethionine, capsaicin, were rationally selected. Screening of the selected desired mutants were carried out by submerged cultivation followed by extraction and quantification of CoQ10. Atorvastatin resistance marker was found to be a useful tool in selecting high CoQ10 producing mutants. EMS induced atorvastatin resistant mutant strain EA22 generated from Sporidiobolus johnsonii ATCC 20490 showed two-fold increase in CoQ10 titer. The mutant strain was morphologically different from parent strain in terms of colony size, pigmentation on agar and showed marked differences in the shape and size of yeast cells as seen from Scanning Electron Microscopic (SEM) observation, indicating phenotypic changes.
Index Terms—Sporidiobolus johnsonii, Coenzyme Q10,Mutant, SEM, EMS, UV.
Prafull Ranadive and Saji George are with Natural Products Department,Fermentation Technology Lab, Piramal Life Sciences Ltd, Nirlon Complex,Off Western Express Highway, Goregaon(East), Mumbai-400063,India.
Alka Mehta is with School of Bio Sciences and Technology, VITUniversity, Vellore-632014, Tamil Nadu, IndiaCorresponding Author:Email: prafull.ranadive@piramal.com
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Cite: Prafull Ranadive, Alka Mehta, and Saji George, "Strain improvement of Sporidiobolus johnsonii -ATCC 20490 for biotechnological production of Coenzyme Q10," International Journal of Chemical Engineering and Applications vol. 2, no. 3, pp. 216-220 , 2011.
