Abstract—The objective of this research is to treat municipal wastewater by barium alginate (BA)-entrapped activated sludge. This study divided into 2 parts including 1) adjustment of operating conditions for municipal wastewater treatment by the BA-entrapped cells in batch experiment and 2) utilization of the BA-entrapped cells in sequencing batch experiment. In the batch experiment, optimization of cell entrapment preparation condition (cell-to-matrix (BA) ratios of 1:5, 1:10, and 1:20 by volume), determination of optimum dissolved oxygen (DO) concentration (DOs of 2, 4, and 6 mg/L), and determination of optimum entrapped cell loading (1,000, 3,000, and 5,000 mg dry cells/L) were performed. The result showed that the cell-to-matrix ratio, DO concentration, and cell loading of 1:10, 6 mg/L, and 1,000 mg/L, respectively performed highest treatability (COD removal of 47-86%). In the sequencing batch mode experiment, the free and BA-entrapped activated sludge reactors operated for 30 runs were performed and microscopically observed. The entrapped cells could treat wastewater for 54% while the free cells treated wastewater of 33%. The micro-structural observation indicated that the BA-entrapped cells slightly damaged after utilization and numerous microbial cells perforated all over the matrix.
Index Terms—Barium alginate, Cell entrapment, Cell-to-matrix ratio, Cell loading, Dissolved oxygen, Municipalwastewater.
S. Siripattanakul-Ratpukdi is with Department of Chemical Engineering,Faculty of Engineering, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand and Center of Excellence for Environmental and Hazardous Waste Management, Bangkok 10330, Thailand (e-mail:ensumasi@ubu.ac.th; jeans_sumana@yahoo.com).
T. Tongkliang is with Department of Chemical Engineering, Faculty of Engineering, Ubon Ratchathani University, Ubon Ratchathani 34190,Thailand (e-mail: sakuya_i@hotmail.co.th).
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Cite: Sumana Siripattanakul-Ratpukdi and Thitiporn Tongkliang, "Municipal Wastewater Treatment Using Barium Alginate Entrapped Activated Sludge: Adjustment of Utilization Conditions," International Journal of Chemical Engineering and Applications vol. 3, no. 5, pp.328-332, 2012.
