Abstract—The increasing demands of consumers for using less chemical preservatives has led to increasing number of researches to produce natural compounds with antimicrobial properties. Fresh aerial parts of wild Ziziphora tenuior were collected in Eastern Iran. Z.tenuior samples were collected after initial preparation isolated from the aboveground plant parts and plant samples preparation by water distillation using Clevenger apparatus after determining the optimal conditions for making essential oil was obtained. Essential oil with an efficiency of 1/1 percent (g/g) was obtained and by GC-MS to determine the composition of the essential oils was analyzed and indicates 21 compounds. Key combinations were as: pulegone (82.6 %) and limonene (6.8%) which was extracted. the studied essential oil would exhibit good antimicrobial properties, this essential oil in 0.08 ml concentration could eliminate microorganisms in water. but this was not evaluated and further investigation should be carried out for use of this essential oil in various industries.
Index Terms—Ziziphora tenuior, antimicrobial, essential oil, GC-MS, heterotrophic plate counts method.
A. Ganjali is with the Department of Experimental Science, Islamic Azad University, Kahnooj Branch, Kahnooj, Iran (e-mail: Amin.Ganjali@ kahnoojiau.ac.ir).
M. Pourramezani Harati is with Young Researchers and Elite Club, Kahnooj Branch, Islamic Azad University, Kahnooj, Iran (e-mail: Maryam. Pourramazani@gmail.com).
M. Kaykhaii is with Department of Chemistry, University of Sistan and Baluchestan, Zahedan, Iran (e-mail: kaykhaii@hamoon.usb.ac.ir).
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Cite: Amin Ganjali, Maryam Pourramezani Harati, and Massoud Kaykhaii, "Antimicrobial Effect of Essential Oil of Ziziphora tenuior on Water by Heterotrophic Plate Counts Method in Kerman (Southeast of Iran)," International Journal of Chemical Engineering and Applications vol. 7, no. 2, pp. 120-123, 2016.
